Contribution of spoligotyping and MIRU-VNTRs to characterize prevalent Mycobacterium tuberculosis genotypes infecting tuberculosis patients in Morocco.
Identifieur interne : 000199 ( Main/Exploration ); précédent : 000198; suivant : 000200Contribution of spoligotyping and MIRU-VNTRs to characterize prevalent Mycobacterium tuberculosis genotypes infecting tuberculosis patients in Morocco.
Auteurs : Imane Chaoui [Maroc] ; Thierry Zozio [France] ; Ouafae Lahlou [Maroc] ; Radia Sabouni [Maroc] ; Mohammed Abid [Maroc] ; Rajae El Aouad [Maroc] ; Mohammed Akrim [Maroc] ; Said Amzazi [Maroc] ; Nalin Rastogi [France] ; Mohammed El Mzibri [Maroc]Source :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [ 1567-7257 ] ; 2014.
Descripteurs français
- KwdFr :
- Adolescent (MeSH), Adulte (MeSH), Adulte d'âge moyen (MeSH), Antibactériens (pharmacologie), Femelle (MeSH), Génome bactérien (MeSH), Humains (MeSH), Jeune adulte (MeSH), Maroc (épidémiologie), Mycobacterium tuberculosis (classification), Mycobacterium tuberculosis (effets des médicaments et des substances chimiques), Mycobacterium tuberculosis (génétique), Mâle (MeSH), Phylogenèse (MeSH), Phylogéographie (MeSH), Répétitions minisatellites (MeSH), Sujet âgé (MeSH), Sujet âgé de 80 ans ou plus (MeSH), Techniques de typage bactérien (méthodes), Tuberculose (microbiologie), Tuberculose (épidémiologie).
- MESH :
- effets des médicaments et des substances chimiques : Mycobacterium tuberculosis.
- génétique : Mycobacterium tuberculosis.
- microbiologie : Tuberculose.
- méthodes : Techniques de typage bactérien.
- pharmacologie : Antibactériens, Mycobacterium tuberculosis.
- épidémiologie : Maroc, Tuberculose.
- Adolescent, Adulte, Adulte d'âge moyen, Femelle, Génome bactérien, Humains, Jeune adulte, Mâle, Phylogenèse, Phylogéographie, Répétitions minisatellites, Sujet âgé, Sujet âgé de 80 ans ou plus.
- Wicri :
- geographic : Maroc.
English descriptors
- KwdEn :
- Adolescent (MeSH), Adult (MeSH), Aged (MeSH), Aged, 80 and over (MeSH), Anti-Bacterial Agents (pharmacology), Bacterial Typing Techniques (methods), Female (MeSH), Genome, Bacterial (MeSH), Humans (MeSH), Male (MeSH), Middle Aged (MeSH), Minisatellite Repeats (MeSH), Morocco (epidemiology), Mycobacterium tuberculosis (classification), Mycobacterium tuberculosis (drug effects), Mycobacterium tuberculosis (genetics), Phylogeny (MeSH), Phylogeography (MeSH), Tuberculosis (epidemiology), Tuberculosis (microbiology), Young Adult (MeSH).
- MESH :
- chemical , pharmacology : Anti-Bacterial Agents.
- geographic , epidemiology : Morocco.
- classification : Mycobacterium tuberculosis.
- drug effects : Mycobacterium tuberculosis.
- epidemiology : Tuberculosis.
- genetics : Mycobacterium tuberculosis.
- methods : Bacterial Typing Techniques.
- microbiology : Tuberculosis.
- Adolescent, Adult, Aged, Aged, 80 and over, Female, Genome, Bacterial, Humans, Male, Middle Aged, Minisatellite Repeats, Phylogeny, Phylogeography, Young Adult.
Abstract
In the present study, Mycobacterium tuberculosis complex (MTBC) clinical isolates from culture-positive TB patients in Morocco were studied by spoligotyping and 12-loci MIRU-VNTR typing methods to characterize prevalent genotypes (n = 219 isolates from 208 patients). Spoligotyping resulted in 39 unique patterns and 167 strains in 30 clusters (2-50 strains per cluster). Comparison with international database showed that 29 of 39 unique patterns matched existing shared spoligotype international types (SITs). Nine shared types containing 10 strains were newly created (SIT 2891 to SIT 2899); this led to the description of 69 SITs with 206 strains and two orphan patterns. The most prevalent spoligotype was SIT42 (LAM; n = 50 or 24% of isolates). The repartition of strains according to major MTBC clades was as follows LAM (46.1%)> Haarlem (26%) >ill-defined T superfamily (22.6%) and S clade (0.96%). On the other hand, Beijing, CAS (Central Asian) and EAI (East-African Indian) strains were absent in this setting. Subsequent 12-Loci MIRU typing resulted in a total of 25 SIT/MIT clusters (n = 66 isolates, 2-6 isolates per cluster), with a resulting recent transmission rate of 22.3%. The MIRU-VNTR patterns corresponded to 69 MITs for 138 strains and 46 orphan patterns. The most frequent patterns were MIT43 (n = 8), MIT9 (n = 7) and MIT42 (n = 7). HGDI analysis of the 12 MIRU loci showed that loci 10, 23 and 40 were highly discriminative in our setting. The results also underlined the usefulness of spoligotyping and MIRU-VNTR to detect mixed infections among certain of our TB patients. Globally, the results obtained showed that TB is almost exclusively transmitted in Morocco through evolutionary-modern MTBC lineages belonging to principal genetic groups 2/3 strains (Haarlem, LAM, T), with a high level of biodiversity seen by MIRU typing. This study provides with a 1st global snapshot of MTBC population structure in Morocco, and validates the potential use of spoligotyping in conjunction with minisatellites for future investigations in Morocco that should in future ideally include optimized 15- or 24-loci MIRU-VNTRs.
DOI: 10.1016/j.meegid.2013.05.023
PubMed: 23732366
Affiliations:
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Electronic address: im_chaoui@yahoo.fr.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Unité de Biologie et Recherches Médicales, Centre National de l'Energie, des Sciences et Techniques Nucléaires, Rabat</wicri:regionArea><placeName><settlement type="city">Rabat</settlement><region nuts="2">Rabat-Salé-Kénitra</region></placeName></affiliation></author><author><name sortKey="Zozio, Thierry" sort="Zozio, Thierry" uniqKey="Zozio T" first="Thierry" last="Zozio">Thierry Zozio</name><affiliation wicri:level="1"><nlm:affiliation>WHO Supranational TB Reference Laboratory, Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, F97183 Abymes, Guadeloupe, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>WHO Supranational TB Reference Laboratory, Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, F97183 Abymes, Guadeloupe</wicri:regionArea><wicri:noRegion>Guadeloupe</wicri:noRegion><wicri:noRegion>Guadeloupe</wicri:noRegion></affiliation></author><author><name sortKey="Lahlou, Ouafae" sort="Lahlou, Ouafae" uniqKey="Lahlou O" first="Ouafae" last="Lahlou">Ouafae Lahlou</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Référence de la Tuberculose, Institut National d'Hygiène, Rabat, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Laboratoire de Référence de la Tuberculose, Institut National d'Hygiène, Rabat</wicri:regionArea><placeName><settlement type="city">Rabat</settlement><region nuts="2">Rabat-Salé-Kénitra</region></placeName></affiliation></author><author><name sortKey="Sabouni, Radia" sort="Sabouni, Radia" uniqKey="Sabouni R" first="Radia" last="Sabouni">Radia Sabouni</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Référence de la Tuberculose, Institut National d'Hygiène, Rabat, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Laboratoire de Référence de la Tuberculose, Institut National d'Hygiène, Rabat</wicri:regionArea><placeName><settlement type="city">Rabat</settlement><region nuts="2">Rabat-Salé-Kénitra</region></placeName></affiliation></author><author><name sortKey="Abid, Mohammed" sort="Abid, Mohammed" uniqKey="Abid M" first="Mohammed" last="Abid">Mohammed Abid</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de génétiques des mycobactéries, service de Recherches, Institut Pasteur, Tanger, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Laboratoire de génétiques des mycobactéries, service de Recherches, Institut Pasteur, Tanger</wicri:regionArea><wicri:noRegion>Tanger</wicri:noRegion></affiliation></author><author><name sortKey="El Aouad, Rajae" sort="El Aouad, Rajae" uniqKey="El Aouad R" first="Rajae" last="El Aouad">Rajae El Aouad</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Référence de la Tuberculose, Institut National d'Hygiène, Rabat, Morocco; Laboratoire de biologie moléculaire, Institut National d'Hygiène, Rabat, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Laboratoire de Référence de la Tuberculose, Institut National d'Hygiène, Rabat, Morocco; Laboratoire de biologie moléculaire, Institut National d'Hygiène, Rabat</wicri:regionArea><placeName><settlement type="city">Rabat</settlement><region nuts="2">Rabat-Salé-Kénitra</region></placeName></affiliation></author><author><name sortKey="Akrim, Mohammed" sort="Akrim, Mohammed" uniqKey="Akrim M" first="Mohammed" last="Akrim">Mohammed Akrim</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de biologie moléculaire, Institut National d'Hygiène, Rabat, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Laboratoire de biologie moléculaire, Institut National d'Hygiène, Rabat</wicri:regionArea><placeName><settlement type="city">Rabat</settlement><region nuts="2">Rabat-Salé-Kénitra</region></placeName></affiliation></author><author><name sortKey="Amzazi, Said" sort="Amzazi, Said" uniqKey="Amzazi S" first="Said" last="Amzazi">Said Amzazi</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de biochimie et Immunologie, Faculté des Sciences, Université Mohammed V, Rabat, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Laboratoire de biochimie et Immunologie, Faculté des Sciences, Université Mohammed V, Rabat</wicri:regionArea><placeName><settlement type="city">Rabat</settlement><region nuts="2">Rabat-Salé-Kénitra</region></placeName></affiliation></author><author><name sortKey="Rastogi, Nalin" sort="Rastogi, Nalin" uniqKey="Rastogi N" first="Nalin" last="Rastogi">Nalin Rastogi</name><affiliation wicri:level="1"><nlm:affiliation>WHO Supranational TB Reference Laboratory, Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, F97183 Abymes, Guadeloupe, France. Electronic address: nrastogi@pasteur-guadeloupe.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>WHO Supranational TB Reference Laboratory, Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, F97183 Abymes, Guadeloupe</wicri:regionArea><wicri:noRegion>Guadeloupe</wicri:noRegion><wicri:noRegion>Guadeloupe</wicri:noRegion></affiliation></author><author><name sortKey="El Mzibri, Mohammed" sort="El Mzibri, Mohammed" uniqKey="El Mzibri M" first="Mohammed" last="El Mzibri">Mohammed El Mzibri</name><affiliation wicri:level="3"><nlm:affiliation>Unité de Biologie et Recherches Médicales, Centre National de l'Energie, des Sciences et Techniques Nucléaires, Rabat, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Unité de Biologie et Recherches Médicales, Centre National de l'Energie, des Sciences et Techniques Nucléaires, Rabat</wicri:regionArea><placeName><settlement type="city">Rabat</settlement><region nuts="2">Rabat-Salé-Kénitra</region></placeName></affiliation></author></analytic><series><title level="j">Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</title><idno type="eISSN">1567-7257</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent (MeSH)</term><term>Adult (MeSH)</term><term>Aged (MeSH)</term><term>Aged, 80 and over (MeSH)</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Bacterial Typing Techniques (methods)</term><term>Female (MeSH)</term><term>Genome, Bacterial (MeSH)</term><term>Humans (MeSH)</term><term>Male (MeSH)</term><term>Middle Aged (MeSH)</term><term>Minisatellite Repeats (MeSH)</term><term>Morocco (epidemiology)</term><term>Mycobacterium tuberculosis (classification)</term><term>Mycobacterium tuberculosis (drug effects)</term><term>Mycobacterium tuberculosis (genetics)</term><term>Phylogeny (MeSH)</term><term>Phylogeography (MeSH)</term><term>Tuberculosis (epidemiology)</term><term>Tuberculosis (microbiology)</term><term>Young Adult (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent (MeSH)</term><term>Adulte (MeSH)</term><term>Adulte d'âge moyen (MeSH)</term><term>Antibactériens (pharmacologie)</term><term>Femelle (MeSH)</term><term>Génome bactérien (MeSH)</term><term>Humains (MeSH)</term><term>Jeune adulte (MeSH)</term><term>Maroc (épidémiologie)</term><term>Mycobacterium tuberculosis (classification)</term><term>Mycobacterium tuberculosis (effets des médicaments et des substances chimiques)</term><term>Mycobacterium tuberculosis (génétique)</term><term>Mâle (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Phylogéographie (MeSH)</term><term>Répétitions minisatellites (MeSH)</term><term>Sujet âgé (MeSH)</term><term>Sujet âgé de 80 ans ou plus (MeSH)</term><term>Techniques de typage bactérien (méthodes)</term><term>Tuberculose (microbiologie)</term><term>Tuberculose (épidémiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Morocco</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Mycobacterium tuberculosis</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Mycobacterium tuberculosis</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Mycobacterium tuberculosis</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Tuberculosis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycobacterium tuberculosis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mycobacterium tuberculosis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Bacterial Typing Techniques</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Tuberculose</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Tuberculosis</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Techniques de typage bactérien</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term><term>Mycobacterium tuberculosis</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Maroc</term><term>Tuberculose</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Female</term><term>Genome, Bacterial</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Minisatellite Repeats</term><term>Phylogeny</term><term>Phylogeography</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Femelle</term><term>Génome bactérien</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Phylogenèse</term><term>Phylogéographie</term><term>Répétitions minisatellites</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Maroc</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the present study, Mycobacterium tuberculosis complex (MTBC) clinical isolates from culture-positive TB patients in Morocco were studied by spoligotyping and 12-loci MIRU-VNTR typing methods to characterize prevalent genotypes (n = 219 isolates from 208 patients). Spoligotyping resulted in 39 unique patterns and 167 strains in 30 clusters (2-50 strains per cluster). Comparison with international database showed that 29 of 39 unique patterns matched existing shared spoligotype international types (SITs). Nine shared types containing 10 strains were newly created (SIT 2891 to SIT 2899); this led to the description of 69 SITs with 206 strains and two orphan patterns. The most prevalent spoligotype was SIT42 (LAM; n = 50 or 24% of isolates). The repartition of strains according to major MTBC clades was as follows LAM (46.1%)> Haarlem (26%) >ill-defined T superfamily (22.6%) and S clade (0.96%). On the other hand, Beijing, CAS (Central Asian) and EAI (East-African Indian) strains were absent in this setting. Subsequent 12-Loci MIRU typing resulted in a total of 25 SIT/MIT clusters (n = 66 isolates, 2-6 isolates per cluster), with a resulting recent transmission rate of 22.3%. The MIRU-VNTR patterns corresponded to 69 MITs for 138 strains and 46 orphan patterns. The most frequent patterns were MIT43 (n = 8), MIT9 (n = 7) and MIT42 (n = 7). HGDI analysis of the 12 MIRU loci showed that loci 10, 23 and 40 were highly discriminative in our setting. The results also underlined the usefulness of spoligotyping and MIRU-VNTR to detect mixed infections among certain of our TB patients. Globally, the results obtained showed that TB is almost exclusively transmitted in Morocco through evolutionary-modern MTBC lineages belonging to principal genetic groups 2/3 strains (Haarlem, LAM, T), with a high level of biodiversity seen by MIRU typing. This study provides with a 1st global snapshot of MTBC population structure in Morocco, and validates the potential use of spoligotyping in conjunction with minisatellites for future investigations in Morocco that should in future ideally include optimized 15- or 24-loci MIRU-VNTRs.</div></front></TEI><affiliations><list><country><li>France</li><li>Maroc</li></country><region><li>Rabat-Salé-Kénitra</li></region><settlement><li>Rabat</li></settlement></list><tree><country name="Maroc"><region name="Rabat-Salé-Kénitra"><name sortKey="Chaoui, Imane" sort="Chaoui, Imane" uniqKey="Chaoui I" first="Imane" last="Chaoui">Imane Chaoui</name></region><name sortKey="Abid, Mohammed" sort="Abid, Mohammed" uniqKey="Abid M" first="Mohammed" last="Abid">Mohammed Abid</name><name sortKey="Akrim, Mohammed" sort="Akrim, Mohammed" uniqKey="Akrim M" first="Mohammed" last="Akrim">Mohammed Akrim</name><name sortKey="Amzazi, Said" sort="Amzazi, Said" uniqKey="Amzazi S" first="Said" last="Amzazi">Said Amzazi</name><name sortKey="El Aouad, Rajae" sort="El Aouad, Rajae" uniqKey="El Aouad R" first="Rajae" last="El Aouad">Rajae El Aouad</name><name sortKey="El Mzibri, Mohammed" sort="El Mzibri, Mohammed" uniqKey="El Mzibri M" first="Mohammed" last="El Mzibri">Mohammed El Mzibri</name><name sortKey="Lahlou, Ouafae" sort="Lahlou, Ouafae" uniqKey="Lahlou O" first="Ouafae" last="Lahlou">Ouafae Lahlou</name><name sortKey="Sabouni, Radia" sort="Sabouni, Radia" uniqKey="Sabouni R" first="Radia" last="Sabouni">Radia Sabouni</name></country><country name="France"><noRegion><name sortKey="Zozio, Thierry" sort="Zozio, Thierry" uniqKey="Zozio T" first="Thierry" last="Zozio">Thierry Zozio</name></noRegion><name sortKey="Rastogi, Nalin" sort="Rastogi, Nalin" uniqKey="Rastogi N" first="Nalin" last="Rastogi">Nalin Rastogi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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